Procedure I – Enzyme Reaction Rate – Concentration Dependence

Procedure I – Enzyme Reaction Rate – Concentration Dependence

Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)

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Trial Concentration

Level

Oxygen

Concentration

Change Data (ppt)

Elapsed

Time

(s)

Enzyme

Reaction Rate

(ppt/s)

1 2.5% 5.17 ppt 20.0 0.2585
2 5.0% 10.34 ppt 20.0 0.517
3 10% 20.68 ppt 20.0 1.034

Observations and Questions

[1] Sample Calculation: Show your enzyme reaction rate calculation for Trial 1.

[2] Convert enzyme concentrations of 2.5%, 5.0%, and 10% to ppt units.

[3] For Procedure I/Table I, identify:

Independent variable: Oxygen Concentration

Dependent variable: Enzyme Reaction Rate

Control variables: Elapsed Time

[4] Draw a graph of the independent variable on the x-axis (abscissa) and the dependent variable on the y-axis (ordinate). Label the axis titles and units.

Procedure II – Enzyme Reaction Rate – Temperature Dependence

Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)

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Trial Temperature

(C)

Oxygen

Concentration

Change Data (ppt)

Elapsed

Time

(s)

Enzyme

Reaction Rate

(ppt/s)

1 20.0 7.29 ppt 20.0
2 30.0 14.67 ppt 20.0
3 55.0 2.29 ppt 20.0

Observations and Questions

[5] Sample Calculation: Show your enzyme reaction rate calculation for Trial 1.

[6] Using the data you reported in the Table above, describe the effect of temperature on the reaction rate.

Procedure III – Enzyme Reaction Rate – pH Dependence

Complete the table below using your data and information found under the Background tab (see the Summary of Needed Formulas section)

Trial pH

Level

Oxygen

Concentration

Change Data (ppt)

Elapsed

Time

(s)

Enzyme

Reaction Rate

(ppt/s)

1 6.0 6.28 ppt 20.0
2 7.0 10.34 ppt 20.0
3 9.0 1.40 ppt 20.0

Observations and Questions

[7] Sample Calculation: Show your enzyme reaction rate calculation for Trial 1.

[8] Choose either a bar graph or a line graph format and plot the data for Procedures II and III. Plot the data for each Procedure on a separate plot.

[9] Compare the plot of the data for Procedures I, II, and III. Describe the differences among the three plots.

[10] What conclusions can draw about the effects of concentration, temperature and pH on the reaction rate after comparing the plots from the three different Procedures?

[11] Choose one of the factors that impacts reaction rate (concentration, temperature, or pH) and, in your own words, provide a plausible biological explanation for your experimental results for that factor.

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